Publications
Gillman C, Nicolas WJ, Martynowycz MW, Gonen T. Design and implementation of suspended drop crystallization. bioRxiv. 2023 Mar 28.
Martynowycz MW, Shiriaeva A, Clabbers MT, Nicolas WJ, Weaver SJ, Hattne J, Gonen T. A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals. Nature Communications. 2023 Feb 25;14(1):1086.
Haymaker A, Bardin AA, Gonen T, Martynowycz MW*, Nannenga BL*. Structure determination of a DNA crystal by MicroED. bioRxiv. 2023:2023-04.
Clabbers MT, Martynowycz MW, Hattne J, Nannenga BL, Gonen T. Electron-counting MicroED data with the K2 and K3 direct electron detectors. Journal of Structural Biology. 2022 Dec 1;214(4):107886.
Dayeen FR, Brandner BA, Martynowycz MW, Kucuk K, Foody MJ, Bu W, Hall SB, Gidalevitz D. Effects of cholesterol on the structure and collapse of DPPC monolayers. Biophysical Journal. 2022 Sep 20;121(18):3533-41.
Martynowycz MW, Gonen T. MICROCRYSTAL ELECTRON DIFFRACTION. Physics Today. 2022 Jun;75:6-38.
Martynowycz MW, Clabbers MT, Hattne J, Gonen T. Ab initio phasing macromolecular structures using electron-counted MicroED data. Nature Methods. 2022 Jun;19(6):724-9.
Gonen T, Martynowycz MW, Hattne J, inventors; Howard Hughes Medical Institute, assignee. Methods and compositions for micro-electron diffraction. United States patent US 11,293,883. 2022 Apr 5.
Clabbers MT, Martynowycz MW, Hattne J, Gonen T. Hydrogens and hydrogen-bond networks in macromolecular MicroED data. Journal of Structural Biology: X. 2022 Jan 1;6:100078.
Martynowycz MW, Clabbers MT, Unge J, Hattne J, Gonen T. Benchmarking the ideal sample thickness in cryo-EM. Proceedings of the National Academy of Sciences. 2021 Dec 7;118(49):e2108884118.
Martynowycz MW, Gonen T. Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED). STAR protocols. 2021 Sep 17;2(3):100686.
Martynowycz MW, Shiriaeva A, Ge X, Hattne J, Nannenga BL, Cherezov V, Gonen T. MicroED structure of the human adenosine receptor determined from a single nanocrystal in LCP. Proceedings of the National Academy of Sciences. 2021 Sep 7;118(36):e2106041118.
Martynowycz MW, Gonen T. Microcrystal Electron Diffraction of Small Molecules. JoVE (Journal of Visualized Experiments). 2021 Mar 15(169):e62313.
Halaby S, Martynowycz MW, Zhu Z, Tretiak S, Zhugayevych A, Gonen T, Seifrid M. Microcrystal Electron Diffraction for Molecular Design of Functional Non-Fullerene Acceptor Structures. Chemistry of Materials. 2021 Jan 25;33(3):966-77.
Martynowycz MW, Gonen T. Ligand incorporation into protein microcrystals for MicroED by on-grid soaking. Structure. 2021 Jan 7;29(1):88-95.
Martynowycz MW, Gonen T. Studying membrane protein structures by MicroED. Structure and Function of Membrane Proteins. 2021:137-51.
Martynowycz MW, Khan F, Hattne J, Abramson J, Gonen T. MicroED structure of lipid-embedded mammalian mitochondrial voltage-dependent anion channel. Proceedings of the National Academy of Sciences. 2020 Dec 22;117(51):32380-5.
Andreev K, Martynowycz MW, Kuzmenko I, Bu W, Hall SB, Gidalevitz D. Structural changes in films of pulmonary surfactant induced by surfactant vesicles. Langmuir. 2020 Oct 20;36(45):13439-47.
Richards LS, Millán C, Miao J, Martynowycz MW, Sawaya MR, Gonen T, Borges RJ, Usón I, Rodriguez JA. Fragment-based determination of a proteinase K structure from MicroED data using ARCIMBOLDO_SHREDDER. Acta Crystallographica Section D: Structural Biology. 2020 Aug 1;76(8):703-12.
Martynowycz MW, Hattne J, Gonen T. Experimental phasing of MicroED data using radiation damage. Structure. 2020 Apr 7;28(4):458-64.
Wolff AM, Young ID, Sierra RG, Brewster AS, Martynowycz MW, Nango E, Sugahara M, Nakane T, Ito K, Aquila A, Bhowmick A. Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals. IUCrJ. 2020 Mar 1;7(2):306-23.
Dick M, Sarai NS, Martynowycz MW, Gonen T, Arnold FH. Tailoring tryptophan synthase TrpB for selective quaternary carbon bond formation. Journal of the American Chemical Society. 2019 Nov 21;141(50):19817-22.
Martynowycz MW, Zhao W, Hattne J, Jensen GJ, Gonen T. Qualitative analyses of polishing and precoating FIB milled crystals for MicroED. Structure. 2019 Oct 1;27(10):1594-600.
Hattne J, Martynowycz MW, Penczek PA, Gonen T. MicroED with the Falcon III direct electron detector. IUCrJ. 2019 Sep 1;6(5):921-6.
Andreev K, Martynowycz MW, Gidalevitz D. Peptoid drug discovery and optimization via surface X‐ray scattering. Biopolymers. 2019 Jun;110(6):e23274.
de la Cruz MJ, Martynowycz MW, Hattne J, Gonen T. MicroED data collection with SerialEM. Ultramicroscopy. 2019 Jun 1;201:77-80.
Martynowycz MW, Rice A, Andreev K, Nobre TM, Kuzmenko I, Wereszczynski J, Gidalevitz D. Salmonella membrane structural remodeling increases resistance to antimicrobial peptide LL-37. ACS infectious diseases. 2019 May 14;5(7):1214-22.
Martynowycz MW, Zhao W, Hattne J, Jensen GJ, Gonen T. Collection of continuous rotation MicroED data from ion beam-milled crystals of any size. Structure. 2019 Mar 5;27(3):545-8.
Jones CG, Martynowycz MW, Hattne J, Fulton TJ, Stoltz BM, Rodriguez JA, Nelson HM, Gonen T. The CryoEM method MicroED as a powerful tool for small molecule structure determination. ACS Central Science. 2018 Nov 2;4(11):1587-92.
Sowah-Kuma D, Fransishyn KM, Cayabyab C, Martynowycz MW, Kuzmenko I, Paige MF. Molecular-level structure and packing in phase-separated Arachidic acid–perfluorotetradecanoic acid monolayer films. Langmuir. 2018 Aug 13;34(36):10673-83.
Andreev K, Martynowycz MW, Huang ML, Kuzmenko I, Bu W, Kirshenbaum K, Gidalevitz D. Hydrophobic interactions modulate antimicrobial peptoid selectivity towards anionic lipid membranes. Biochimica et Biophysica Acta (BBA)-Biomembranes. 2018 Jun 1;1860(6):1414-23.
Hattne J, Shi D, Glynn C, Zee CT, Gallagher-Jones M, Martynowycz MW, Rodriguez JA, Gonen T. Analysis of global and site-specific radiation damage in cryo-EM. Structure. 2018 May 1;26(5):759-66.
Martynowycz MW, Gonen T. From electron crystallography of 2D crystals to MicroED of 3D crystals. Current opinion in colloid & interface science. 2018 Mar 1;34:9-16.
Gallagher-Jones M, Glynn C, Boyer DR, Martynowycz MW, Hernandez E, Miao J, Zee CT, Novikova IV, Goldschmidt L, McFarlane HT, Helguera GF. Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp. Nature structural & molecular biology. 2018 Feb;25(2):131-4.
Vergara S, Lukes DA, Martynowycz MW, Santiago U, Plascencia-Villa G, Weiss SC, de La Cruz MJ, Black DM, Alvarez MM, Lopez-Lozano X, Barnes CO. MicroED structure of Au146 (p-MBA) 57 at subatomic resolution reveals a twinned FCC cluster. The journal of physical chemistry letters. 2017 Nov 16;8(22):5523-30.
Andreev K, Martynowycz MW, Ivankin A, Huang ML, Kuzmenko I, Meron M, Lin B, Kirshenbaum K, Gidalevitz D. Cyclization improves membrane permeation by antimicrobial peptoids. Langmuir. 2016 Dec 6;32(48):12905-13.
Martynowycz MW, Hu B, Kuzmenko I, Bu W, Hock A, Gidalevitz D. Monomolecular siloxane film as a model of single site catalysts. Journal of the American Chemical Society. 2016 Sep 28;138(38):12432-9.
Feinstein DL, Akpa BS, Ayee MA, Boullerne AI, Braun D, Brodsky SV, Gidalevitz D, Hauck Z, Kalinin S, Kowal K, Kuzmenko I. The emerging threat of superwarfarins: history, detection, mechanisms, and countermeasures. Annals of the New York Academy of Sciences. 2016 Jun;1374(1):111-22.
Marangoni MN, Martynowycz MW, Kuzmenko I, Braun D, Polak PE, Weinberg G, Rubinstein I, Gidalevitz D, Feinstein DL. Membrane cholesterol modulates superwarfarin toxicity. Biophysical journal. 2016 Apr 26;110(8):1777-88.
Nobre TM, Martynowycz MW, Andreev K, Kuzmenko I, Nikaido H, Gidalevitz D. Modification of Salmonella lipopolysaccharides prevents the outer membrane penetration of novobiocin. Biophysical journal. 2015 Dec 15;109(12):2537-45.